The Ultimate Biohacker's Peptide Stack for Muscle Size & Strength.

The Ultimate Biohacker's Peptide Stack for Muscle Size & Strength.

Building serious muscle size and strength is about far more than lifting heavy weights. It requires precise control over growth pathways, hormonal pulses, recovery systems, nutrient partitioning, and mitochondrial performance. This stack is designed to leave no stone unturned, targeting every relevant biological pathway with high-efficacy peptides—ideal for the performance-obsessed biohacker.

1. Growth Hormone Axis Optimization (Dual Pulse Strategy)

CJC-1295 (no DAC) – GHRH Pathway

• Dose: 100 mcg AM or pre-workout, subQ
• Mechanism: Stimulates the pituitary via the Growth Hormone-Releasing Hormone receptor to produce a natural GH pulse.
• Why It’s Used: Allows for tight control of GH pulses without receptor saturation; mimics physiological GH release.

Ipamorelin – Ghrelin Receptor Pathway

• Dose: 100–300 mcg AM (with CJC) and pre-bed, subQ
• Mechanism: Stimulates GH release via ghrelin receptors without affecting cortisol or prolactin.
• Why It’s Used: Pairs synergistically with CJC and Tesamorelin for potent, controlled GH spikes.

Tesamorelin – Enhanced GHRH Analog

• Dose: 2 mg pre-bed, subQ
• Mechanism: Potent GHRH analog with strong GH-releasing power and FDA backing for visceral fat reduction.
• Why It’s Used: Drives significant GH output overnight during the body’s natural GH peak.

2. Nutrient Partitioning & IGF-1 Elevation

IGF-1 LR3 – IGF-1 Pathway

• Dose: 30–50 mcg post-workout, subQ or IM
• Mechanism: Increases muscle cell proliferation and nutrient uptake via IGF-1 receptor activation.
• Why It’s Used: Directly promotes hypertrophy and improves recovery through enhanced protein synthesis.

AOD-9604 – Fragment of HGH (176-191)

• Dose: 300 mcg AM fasted, subQ
• Mechanism: Targets fat metabolism by mobilizing free fatty acids without suppressing appetite.
• Why It’s Used: Supports energy production and fat burning during mass phases without compromising calorie intake.

3. Mitochondrial Biogenesis & Energy Efficiency

Fat Transport and Oxidation: L-Carnitine ensures that fatty acids are transported into the mitochondria, where they are burned for energy, supported by MOTS-c

5-Amino-1MQ – NNMT Inhibition / NAD+ Modulation

• Dose: 50–100 mg daily, oral
• Mechanism: Inhibits NNMT, increasing NAD+ availability for metabolic and muscular endurance.
• Why It’s Used: Boosts fat oxidation and mitochondrial function for superior gym performance.

MOTS-c – Mitochondrial Peptide / AMPK Activator

• Dose: 5 mg, 3x/week AM or pre-workout, subQ
• Mechanism: Enhances mitochondrial respiration and insulin sensitivity through AMPK activation.
• Why It’s Used: Improves energy efficiency and metabolic flexibility, especially under stress.

SS-31 (Elamipretide) – Mitochondrial Membrane Stabilizer

• Dose: 2–5 mg pre-workout or daily, subQ
• Mechanism: Targets cardiolipin in mitochondria to enhance ATP production and reduce oxidative stress.
• Why It’s Used: Increases muscular endurance and reduces fatigue during high-intensity training.

4. Recovery, Tissue Repair & Inflammation Control

BPC-157 – Angiogenesis and Anti-Inflammatory Pathway

• Dose: 250–500 mcg 2x/day, subQ or oral
• Mechanism: Accelerates healing through angiogenesis and modulation of inflammatory cytokines.
• Why It’s Used: Enhances muscle, tendon, and gut recovery post-training or injury.

TB-500 (Thymosin Beta-4) – Actin Upregulation / Cell Migration

• Dose: 2–5 mg/week, split doses, subQ
• Mechanism: Promotes wound healing, tissue regeneration, and cellular repair.
• Why It’s Used: Supports systemic repair—ideal for hard training phases or injury recovery.

GHK-Cu – Copper Peptide Pathway

• Dose: 1–2 mg daily, subQ or topical
• Mechanism: Promotes collagen synthesis, tissue regeneration, and skin repair.
• Why It’s Used: Boosts muscle, joint, and connective tissue health while improving skin quality.

5. Gut Health & Nutrient Absorption

KPV – Melanocortin Pathway (Anti-inflammatory)

• Dose: 200 mcg daily, oral or subQ
• Mechanism: Downregulates inflammatory cytokines and promotes gut lining integrity.
• Why It’s Used: Improves digestion, absorption, and systemic inflammation control.

Oral BPC-157 – GI Tract Regeneration

• Dose: 250 mcg 2x/day, oral
• Mechanism: Repairs gastrointestinal mucosa, promotes microbiome balance.
• Why It’s Used: Enhances nutrient absorption and recovery, especially important during mass phases.

6. CNS Recovery, Sleep & Motivation

Dihexa – Neurotrophic Modulator (BDNF Pathway)

• Dose: 2–10 mg AM, oral
• Mechanism: Enhances neurogenesis, synaptic plasticity, and motor learning.
• Why It’s Used: Improves focus, motivation, and mental drive for training.

DSIP (Delta Sleep-Inducing Peptide) – Pituitary and Sleep Pathways

• Dose: 100 mcg pre-bed, subQ
• Mechanism: Promotes deep sleep and pituitary recovery.
• Why It’s Used: Enhances rest, recovery, and growth hormone response overnight.

Final Notes for the Biohacker

This stack is for serious users only—those willing to dial in nutrition, sleep, training, and stress management. It supports virtually every system involved in muscular hypertrophy and recovery. Each peptide is chosen for its unique mechanism and ability to synergize with the rest of the stack.

Cycle length, injection timing, and peptide sourcing are critical to success. Always consult a medical professional and use peptides from high-quality, tested sources.

For L-Carnitine, River recommends symbiotelabz.co.uk – Symbiote Labz